| First Author | Bohl JM | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 3 | Pages | 101482 |
| PubMed ID | 35769922 | Mgi Jnum | J:351548 |
| Mgi Id | MGI:7640305 | Doi | 10.1016/j.xpro.2022.101482 |
| Citation | Bohl JM, et al. (2022) Patch clamp recording from bipolar cells in the wholemount mouse retina. STAR Protoc 3(3):101482 |
| abstractText | Bipolar cells are the second-order neurons in the retina that are less accessible for investigating their synaptic responses. Here, we present a protocol to conduct patch clamp recordings from bipolar cells in the wholemount retina from Ai32 mutant mice. We detail whole-cell patch-clamp recording from bipolar cells to examine their light-evoked responses to optogenetic stimulation, followed by imaging terminals of recorded cells to determine bipolar cell type. We describe light stimulus information to activate channelrhodopsin-2 (ChR2). For complete details on the use and execution of this protocol, please refer to Hellmer et al. (2021). |
