| First Author | Patel JC | Year | 2024 |
| Journal | Cell Rep | Volume | 43 |
| Issue | 3 | Pages | 113834 |
| PubMed ID | 38431842 | Mgi Jnum | J:348593 |
| Mgi Id | MGI:7616169 | Doi | 10.1016/j.celrep.2024.113834 |
| Citation | Patel JC, et al. (2024) GABA co-released from striatal dopamine axons dampens phasic dopamine release through autoregulatory GABA(A) receptors. Cell Rep 43(3):113834 |
| abstractText | Striatal dopamine axons co-release dopamine and gamma-aminobutyric acid (GABA), using GABA provided by uptake via GABA transporter-1 (GAT1). Functions of GABA co-release are poorly understood. We asked whether co-released GABA autoinhibits dopamine release via axonal GABA type A receptors (GABA(A)Rs), complementing established inhibition by dopamine acting at axonal D2 autoreceptors. We show that dopamine axons express alpha3-GABA(A)R subunits in mouse striatum. Enhanced dopamine release evoked by single-pulse optical stimulation in striatal slices with GABA(A)R antagonism confirms that an endogenous GABA tone limits dopamine release. Strikingly, an additional inhibitory component is seen when multiple pulses are used to mimic phasic axonal activity, revealing the role of GABA(A)R-mediated autoinhibition of dopamine release. This autoregulation is lost in conditional GAT1-knockout mice lacking GABA co-release. Given the faster kinetics of ionotropic GABA(A)Rs than G-protein-coupled D2 autoreceptors, our data reveal a mechanism whereby co-released GABA acts as a first responder to dampen phasic-to-tonic dopamine signaling. |
