| First Author | Spencer NG | Year | 2017 |
| Journal | MAGMA | Volume | 30 |
| Issue | 2 | Pages | 153-163 |
| PubMed ID | 27785640 | Mgi Jnum | J:350192 |
| Mgi Id | MGI:7661158 | Doi | 10.1007/s10334-016-0593-9 |
| Citation | Spencer NG, et al. (2017) Can MRI T(1) be used to detect early changes in 5xFAD Alzheimer's mouse brain?. MAGMA 30(2):153-163 |
| abstractText | OBJECTIVES: In the present study, we have tested whether MRI T(1) relaxation time is a sensitive marker to detect early stages of amyloidosis and gliosis in the young 5xFAD transgenic mouse, a well-established animal model for Alzheimer's disease. MATERIALS AND METHODS: 5xFAD and wild-type mice were imaged in a 4.7 T Varian horizontal bore MRI system to generate T(1) quantitative maps using the spin-echo multi-slice sequence. Following immunostaining for glial fibrillary acidic protein, Iba-1, and amyloid-beta, T(1) and area fraction of staining were quantified in the posterior parietal and primary somatosensory cortex and corpus callosum. RESULTS: In comparison with age-matched wild-type mice, we observed first signs of amyloidosis in 2.5-month-old 5xFAD mice, and development of gliosis in 5-month-old 5xFAD mice. In contrast, MRI T(1) relaxation times of young, i.e., 2.5- and 5-month-old, 5xFAD mice were not significantly different to those of age-matched wild-type controls. Furthermore, although disease progression was detectable by increased amyloid-beta load in the brain of 5-month-old 5xFAD mice compared with 2.5-month-old 5xFAD mice, MRI T(1) relaxation time did not change. CONCLUSIONS: In summary, our data suggest that MRI T(1) relaxation time is neither a sensitive measure of disease onset nor progression at early stages in the 5xFAD mouse transgenic mouse model. |
