| First Author | Avrahami L | Year | 2013 |
| Journal | J Biol Chem | Volume | 288 |
| Issue | 2 | Pages | 1295-306 |
| PubMed ID | 23155049 | Mgi Jnum | J:193739 |
| Mgi Id | MGI:5469508 | Doi | 10.1074/jbc.M112.409250 |
| Citation | Avrahami L, et al. (2013) Inhibition of glycogen synthase kinase-3 ameliorates beta-amyloid pathology and restores lysosomal acidification and mammalian target of rapamycin activity in the Alzheimer disease mouse model: in vivo and in vitro studies. J Biol Chem 288(2):1295-306 |
| abstractText | Accumulation of beta-amyloid (Abeta) deposits is a primary pathological feature of Alzheimer disease that is correlated with neurotoxicity and cognitive decline. The role of glycogen synthase kinase-3 (GSK-3) in Alzheimer disease pathogenesis has been debated. To study the role of GSK-3 in Abeta pathology, we used 5XFAD mice co-expressing mutated amyloid precursor protein and presenilin-1 that develop massive cerebral Abeta loads. Both GSK-3 isozymes (alpha/beta) were hyperactive in this model. Nasal treatment of 5XFAD mice with a novel substrate competitive GSK-3 inhibitor, L803-mts, reduced Abeta deposits and ameliorated cognitive deficits. Analyses of 5XFAD hemi-brain samples indicated that L803-mts restored the activity of mammalian target of rapamycin (mTOR) and inhibited autophagy. Lysosomal acidification was impaired in the 5XFAD brains as indicated by reduced cathepsin D activity and decreased N-glycoyslation of the vacuolar ATPase subunit V0a1, a modification required for lysosomal acidification. Treatment with L803-mts restored lysosomal acidification in 5XFAD brains. Studies in SH-SY5Y cells confirmed that GSK-3alpha and GSK-3beta impair lysosomal acidification and that treatment with L803-mts enhanced the acidic lysosomal pool as demonstrated in LysoTracker Red-stained cells. Furthermore, L803-mts restored impaired lysosomal acidification caused by dysfunctional presenilin-1. We provide evidence that mTOR is a target activated by GSK-3 but inhibited by impaired lysosomal acidification and elevation in amyloid precursor protein/Abeta loads. Taken together, our data indicate that GSK-3 is a player in Abeta pathology. Inhibition of GSK-3 restores lysosomal acidification that in turn enables clearance of Abeta burdens and reactivation of mTOR. These changes facilitate amelioration in cognitive function. |
