| First Author | Liu L | Year | 2020 |
| Journal | Bio Protoc | Volume | 10 |
| Issue | 19 | Pages | e3771 |
| PubMed ID | 33659429 | Mgi Jnum | J:352912 |
| Mgi Id | MGI:7664384 | Doi | 10.21769/BioProtoc.3771 |
| Citation | Liu L, et al. (2020) Preparing Viable Hippocampal Slices from Adult Mice for the Study of Sharp Wave-ripples. Bio Protoc 10(19):e3771 |
| abstractText | We describe a protocol for preparing acute brain slices which can produce robust hippocampal sharp wave-ripples (SWRs) in vitro. The protocol is optimized for its simplicity and reliability for the preparation of solutions, slicing, and recovery incubation. Most slices in almost every mouse prepared though the protocol expressed vigorous spontaneous SWRs for ~24 h, compared to the 20-30% viability from "standard" low sodium slicing protocols. SWRs are spontaneous neuronal activity in the hippocampus and are essential for consolidation of episodic memory. Brain slices reliably expressing SWRs are useful for studying memory impairment and brain degeneration diseases in ex vivo experiments. Spontaneous expression of SWRs is sensitive to conditions of slicing and perfusion/oxygenation during recording. The amplitude and abundance of SWRs are often used as a biomarker for viable slices. Key improvements include fast circulation, a long recovery period (3-6 h) after slicing, and allowing tissue to recover at 32 degrees C in a well perfused incubation chamber. Slices in our custom-made apparatus can express spontaneous SWRs for many hours, suggesting a long period with balanced excitation and inhibition in the local networks. Slices from older mice (~postnatal 180 days) show similar viability to younger (postnatal 21-30) mice. |
