| First Author | Kislinger G | Year | 2020 |
| Journal | iScience | Volume | 23 |
| Issue | 7 | Pages | 101290 |
| PubMed ID | 32622266 | Mgi Jnum | J:352645 |
| Mgi Id | MGI:7704290 | Doi | 10.1016/j.isci.2020.101290 |
| Citation | Kislinger G, et al. (2020) Multiscale ATUM-FIB Microscopy Enables Targeted Ultrastructural Analysis at Isotropic Resolution. iScience 23(7):101290 |
| abstractText | Volume electron microscopy enables the ultrastructural analysis of biological tissue. Currently, the techniques involving ultramicrotomy (ATUM, ssTEM) allow large fields of view but afford only limited z-resolution, whereas ion beam-milling approaches (FIB-SEM) yield isotropic voxels but are restricted in volume size. Now we present a hybrid method, named ATUM-FIB, which combines the advantages of both approaches. ATUM-FIB is based on serial sectioning of tissue into "semithick" (2-10 mum) sections collected onto tape. Serial light and electron microscopy allows the identification of regions of interest that are then directly accessible for targeted FIB-SEM. The set of semithick sections thus represents a tissue "library" which provides three-dimensional context information that can be probed "on demand" by local high-resolution analysis. We demonstrate the potential of this technique to reveal the ultrastructure of rare but pathologically important events by identifying microglia contact sites with amyloid plaques in a mouse model of familial Alzheimer's disease. |
