| First Author | Kislinger G | Year | 2020 |
| Journal | STAR Protoc | Volume | 1 |
| Issue | 3 | Pages | 100232 |
| PubMed ID | 33377119 | Mgi Jnum | J:349537 |
| Mgi Id | MGI:6727741 | Doi | 10.1016/j.xpro.2020.100232 |
| Citation | Kislinger G, et al. (2020) ATUM-FIB microscopy for targeting and multiscale imaging of rare events in mouse cortex. STAR Protoc 1(3):100232 |
| abstractText | Here, we describe a detailed workflow for ATUM-FIB microscopy, a hybrid method that combines serial-sectioning scanning electron microscopy (SEM) with focused ion beam SEM (FIB-SEM). This detailed protocol is optimized for mouse cortex samples. The main processing steps include the generation of semi-thick sections from sequentially cured resin blocks using a heated microtomy approach. We demonstrate the different imaging modalities, including serial light and electron microscopy for target recognition and FIB-SEM for isotropic imaging of regions of interest. For complete details on the use and execution of this protocol, please refer to Kislinger et al. (2020). |
