Other
17 Authors
- Ren J,
- Luo Q,
- Raymond JL,
- Kolluru SS,
- Friedmann D,
- Li A,
- Wang R,
- Isakova A,
- Luo L,
- Grutzner SM,
- Li P,
- Quake SR,
- Luo M,
- Lin R,
- Pun A,
- Zeng J,
- Zhao GQ
| First Author | Ren J | Year | 2019 |
| Journal | Elife | Volume | 8 |
| PubMed ID | 31647409 | Mgi Jnum | J:283638 |
| Mgi Id | MGI:6376674 | Doi | 10.7554/eLife.49424 |
| Citation | Ren J, et al. (2019) Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei. Elife 8:e49424 |
| abstractText | Serotonin neurons of the dorsal and median raphe nuclei (DR, MR) collectively innervate the entire forebrain and midbrain, modulating diverse physiology and behavior. To gain a fundamental understanding of their molecular heterogeneity, we used plate-based single-cell RNA-sequencing to generate a comprehensive dataset comprising eleven transcriptomically distinct serotonin neuron clusters. Systematic in situ hybridization mapped specific clusters to the principal DR, caudal DR, or MR. These transcriptomic clusters differentially express a rich repertoire of neuropeptides, receptors, ion channels, and transcription factors. We generated novel intersectional viral-genetic tools to access specific subpopulations. Whole-brain axonal projection mapping revealed that DR serotonin neurons co-expressing vesicular glutamate transporter-3 preferentially innervate the cortex, whereas those co-expressing thyrotropin-releasing hormone innervate subcortical regions in particular the hypothalamus. Reconstruction of 50 individual DR serotonin neurons revealed diverse and segregated axonal projection patterns at the single-cell level. Together, these results provide a molecular foundation of the heterogenous serotonin neuronal phenotypes. |
