| First Author | Anderson R | Year | 2000 |
| Journal | Mech Dev | Volume | 91 |
| Issue | 1-2 | Pages | 61-8 |
| PubMed ID | 10704831 | Mgi Jnum | J:134555 |
| Mgi Id | MGI:3789247 | Doi | 10.1016/s0925-4773(99)00271-3 |
| Citation | Anderson R, et al. (2000) The onset of germ cell migration in the mouse embryo. Mech Dev 91(1-2):61-8 |
| abstractText | Mouse primordial germ cells (PGCs) are specified between embryonic day 6.5 (E6.5) and E7.5, when they have been visualized as an alkaline phosphatase-positive (AP+) cell population in the developing allantois. By E8.5, they are embedded in the hind-gut epithelium. Previous experiments have suggested different sites for PGCs' origin, and it is unclear how they reach the gut epithelium. We have used transgenic mice expressing GFP under a truncated Oct4 promoter to visualize living PGCs. We find GFP+/AP+ cells in the posterior end of the primitive streak as a dispersed population of cells actively migrating into the allantois, and directly into the adjacent embryonic endoderm. Time-lapse analysis shows these cells to be actively migratory from the time they exit the primitive streak. |
