| First Author | Esteban A | Year | 2011 |
| Journal | Proc Natl Acad Sci U S A | Volume | 108 |
| Issue | 34 | Pages | 14270-5 |
| PubMed ID | 21825168 | Mgi Jnum | J:175984 |
| Mgi Id | MGI:5288092 | Doi | 10.1073/pnas.1111415108 |
| Citation | Esteban A, et al. (2011) Fungal recognition is mediated by the association of dectin-1 and galectin-3 in macrophages. Proc Natl Acad Sci U S A 108(34):14270-5 |
| abstractText | Dectin-1, the major beta-glucan receptor in leukocytes, triggers an effective immune response upon fungal recognition. Here we use sortase-mediated transpeptidation, a technique that allows placement of a variety of probes on a polypeptide backbone, to monitor the behavior of labeled functional dectin-1 in live cells with and without fungal challenge. Installation of probes on dectin-1 by sortagging permitted highly specific visualization of functional protein on the cell surface and its subsequent internalization upon ligand presentation. Retrieval of sortagged dectin-1 expressed in macrophages uncovered a unique interaction between dectin-1 and galectin-3 that functions in the proinflammatory response of macrophages to pathogenic fungi. When macrophages expressing dectin-1 are exposed to Candida albicans mutants with increased exposure of beta-glucan, the loss of galectin-3 dramatically accentuates the failure to trigger an appropriate TNF-alpha response. |
