| Primary Identifier | MGI:3696658 | Allele Type | Targeted |
| Gene | Krt14 | Transmission | Germline |
| Strain of Origin | 129X1/SvJ | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Sequence encoding the N-terminal head domain in exon 1 was replaced with the sequence for the keratin 10 head domain. Exons 7 and 8 were deleted and a cDNA encoding the keratin 10 tail domain was fused with exon 6 of keratin 14. A pgk-neo flanked by loxP sites was introduced into intron 1 and subsequently removed via cre mediated recombination. Expression of keratin 10 was detected by Western blotting, confirming successful creation of the chimeric protein. |
