| First Author | Uchiumi T | Year | 2006 |
| Journal | J Biol Chem | Volume | 281 |
| Issue | 52 | Pages | 40440-9 |
| PubMed ID | 17082189 | Mgi Jnum | J:117725 |
| Mgi Id | MGI:3697297 | Doi | 10.1074/jbc.M605948200 |
| Citation | Uchiumi T, et al. (2006) YB-1 is important for an early stage embryonic development: neural tube formation and cell proliferation. J Biol Chem 281(52):40440-9 |
| abstractText | The eukaryotic Y-box-binding protein-1 (YB-1) is involved in the transcriptional and translational control of many biological processes, including cell proliferation. In clinical studies, the cellular level of YB-1 closely correlates with tumor growth and prognosis. To understand the role of YB-1 in vivo, especially in the developmental process, we generated YB-1 knock-out mice, which are embryonic lethal and exhibit exencephaly associated with abnormal patterns of cell proliferation within the neuroepithelium. beta-Actin expression and F-actin formation were reduced in the YB-1 null embryo and YB-1(-/-) mouse embryonic fibroblasts, suggesting that the neural tube defect is caused by abnormal cell morphology and actin assembly within the neuroepithelium. Fibroblasts derived from YB-1(-/-) embryos demonstrated reduced growth and cell density. A colony formation assay showed that YB-1(-/-) mouse embryonic fibroblasts failed to undergo morphological transformation and remained contact-inhibited in culture. These results demonstrate that YB-1 is involved in early mouse development, including neural tube closure and cell proliferation. |
