| Primary Identifier | MGI:3700152 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Tg(TERT)C10Hode |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| description | Mice of two independent transgenic founder lines, designated BAC-C2 and BAC-C10, were crossed with mice heterozygous for a targeted mutation of the endogenous mouse Tert gene to produce lines segregating for the transgene and the targeted mutation. Expression analyses were performed on mice of various genotypes from both lines. Most data presented were from line BAC-C10. |
| molecularNote | A linearized bacterial artificial chromosome (BAC) containing the 54-kb human telomerase reverse transcriptase gene, including at least 11 kb of upstream sequence, all of the exons and introns, and 1.2 kb of downstream sequence, was microinjected into C57BL/6 oocytes. Transcript expression patterns of the human gene in most organs of transgenic mice are generally characteristic of TERT expression in humans, rather than of the endogenous mouse gene; the exception is that human TERT, which is not expressed in human brain, is like the mouse enzyme expressed in mouse brain. |
