Primary Identifier | MGI:3700020 | Allele Type | Targeted |
Attribute String | Modified regulatory region, Null/knockout | Gene | Wnt1 |
Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 5.5 kb enhancer element, located 3' to the Wnt1 polyadenylation signal sequence, was replaced with a floxed PGKneo selection cassette by homologous recombination. This cassette was removed by cre recombination via injection of a cre-encoding plasmid into pronuclei of zygotes from heterozygous intercrosses. |