| First Author | Danielian PS | Year | 1997 |
| Journal | Dev Biol | Volume | 192 |
| Issue | 2 | Pages | 300-9 |
| PubMed ID | 9441669 | Mgi Jnum | J:118582 |
| Mgi Id | MGI:3699846 | Doi | 10.1006/dbio.1997.8762 |
| Citation | Danielian PS, et al. (1997) A 5.5-kb enhancer is both necessary and sufficient for regulation of Wnt-1 transcription in vivo. Dev Biol 192(2):300-9 |
| abstractText | Wnt-1 encodes a secreted signaling molecule which is required for development of the midbrain and anterior hindbrain in the mouse. Wnt-1 expression is initiated early in the development of the central nervous system in a region predicted to give rise to the midbrain. Later in development Wnt-1 expression is restricted to regions of the forebrain, midbrain, and spinal cord. Previous studies identified a 5.5-kb enhancer in the Wnt-1 locus which is sufficient to activate transcription of a reporter gene in a pattern very similar to that of the endogenous Wnt-1 gene. Here we have assessed if this enhancer is an important component of the endogenous regulatory sequences of Wnt-1 by gene targeting and by testing if it is able to express Wnt-1 in a pattern which is sufficient to rescue the phenotype of loss of Wnt-1. Our results show that the 5.5-kb enhancer is both necessary and sufficient for Wnt-1 expression in vivo. |
