| Primary Identifier | MGI:3703057 | Allele Type | Transgenic |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A transgenic construct was designed with the CMV enhancer/chicken beta-actin promoter, loxP-flanked STOP sequence (beta-geo reporter followed by SV40 polyadenylation signal), Myc-tagged intracellular portion of human NOTCH1 (IC-Notch), internal ribosomal entry site, and an enhanced green fluorescent protein (EGFP) gene. Prior to cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the intracellular human NOTCH1 (IC-Notch) and EGFP to proceed in the Cre recombinase expressing cells and enabling tissue-specific studies of NOTCH expression using appropriate Cre mice. |
