| First Author | Macpherson T | Year | 2023 |
| Journal | eNeuro | Volume | 10 |
| Issue | 8 | PubMed ID | 37553242 |
| Mgi Jnum | J:341115 | Mgi Id | MGI:7528279 |
| Doi | 10.1523/ENEURO.0236-23.2023 | Citation | Macpherson T, et al. (2023) alpha4-Containing GABA(A) Receptors on DRD2 Neurons of the Nucleus Accumbens Mediate Instrumental Responding for Conditioned Reinforcers and Its Potentiation by Cocaine. eNeuro 10(8):ENEURO.0236-23.2023 |
| abstractText | Extrasynaptic GABA(A) receptors (GABA(A)Rs) composed of alpha4, beta, and delta subunits mediate GABAergic tonic inhibition and are potential molecular targets in the modulation of behavioral responses to natural and drug rewards. These GABA(A)Rs are highly expressed within the nucleus accumbens (NAc), where they influence the excitability of the medium spiny neurons. Here, we explore their role in modulating behavioral responses to food-conditioned cues and the behavior-potentiating effects of cocaine. alpha4-Subunit constitutive knock-out mice (alpha4(-/-)) showed higher rates of instrumental responding for reward-paired stimuli in a test of conditioned reinforcement (CRf). A similar effect was seen following viral knockdown of GABA(A)R alpha4 subunits within the NAc. Local infusion of the alpha4betadelta-GABA(A)R-preferring agonist THIP (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol; Gaboxadol) into the NAc had no effect on responding when given alone but reduced cocaine potentiation of responding for conditioned reinforcers in wild-type, but not alpha4(-/-) mice. Finally, specific deletion of alpha4-subunits from dopamine D2, but not D1, receptor-expressing neurons (DRD2 and DRD1 neurons), mimicked the phenotype of the constitutive knockout, potentiating CRf responding, and blocking intra-accumbal THIP attenuation of cocaine-potentiated CRf responding. These data demonstrate that alpha4-GABA(A)R-mediated inhibition of DRD2 neurons reduces instrumental responding for a conditioned reinforcer and its potentiation by cocaine and emphasize the importance of GABAergic signaling within the NAc in mediating the effects of cocaine. |
