| First Author | Yang DJ | Year | 2022 |
| Journal | Metabolism | Volume | 135 |
| Pages | 155273 | PubMed ID | 35926636 |
| Mgi Jnum | J:327291 | Mgi Id | MGI:7329632 |
| Doi | 10.1016/j.metabol.2022.155273 | Citation | Yang DJ, et al. (2022) Primary cilia regulate adaptive responses to fasting. Metabolism 135:155273 |
| abstractText | OBJECTIVE: Neuronal primary cilia are known to be a required organelle for energy balance and leptin action. However, whether primary cilia directly mediate adaptive responses during starvation is yet unknown. Therefore, we investigated the counterregulatory roles of primary cilia, and their related leptin action in energy-depleted condition. METHOD: We generated leptin receptor (LepR) neuron-specific primary cilia knockout (Ift88 KO(LepR)) mice. Leptin-mediated electrophysiological properties of the neurons in fasting condition were assessed using patch-clamp technique. Adaptive responses and neuroendocrine reflexes were measured by monitoring counterregulatory hormones. RESULTS: In fasting state, the leptin-induced neuronal excitability and leptin homeostasis were impaired in Ift88 KO(LepR). In addition, the Ift88 KO(LepR) exhibited aberrant fasting responses including lesser body weight loss, decreased energy expenditure, and lower heat generation compared to wild-type littermates. Furthermore, the primary cilia in LepR neurons are necessary for counterregulatory responses and leptin-mediated neuroendocrine adaptation to starvation. CONCLUSION: Our results demonstrated that the neuronal primary cilia are crucial neuronal components mediating the adaptive counterregulatory responses to starvation. |
