| Primary Identifier | MGI:3713708 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Fabp4-Plin*)1Asg |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A mouse Plin cDNA that had serine-to-alanine mutations made in all 6 protein kinase A phosphorylation sites using recombinant adenoviruses (Muta-Gene Phagemid in vitro mutagenesis kit (Bio-Rad)). The cDNA was FLAG-tagged at the carboxy terminus via PCR and was placed in a pBluescript vector containing the Fabp2 enhancer/promoter region, the SV40 small tumor antigen splice site, and polyadenylation signal sequence. This construct was injected in C57BL/6 fertilized oocytes. Mice express the transgene almost exclusively in brown adipose tissue. |
