| Primary Identifier | MGI:3714460 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Nkd1 |
| Transmission | Germline | Strain of Origin | 129/SvEv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to insert a stop codon into exon 5 at amino acid 91, as well as replace exons 5-10 of the targeted gene with an internal ribosome entry site-b-galactosidase (IRES-lacZ) fusion gene and neomycin cassette. The construct was electroporated into 129SvEv-derived embryonic stem (ES) cells. Northern blot analysis of lung tissue and Western blot analysis of embronic tissue in homozygotes confirm lack of normal mRNA expression, while X-gal staining closely mimics dynamic expression pattern of normal Nkd1 mRNA. |
