| First Author | Uchida A | Year | 2016 |
| Journal | Biochem Biophys Res Commun | Volume | 476 |
| Issue | 4 | Pages | 546-552 |
| PubMed ID | 27255992 | Mgi Jnum | J:236032 |
| Mgi Id | MGI:5804486 | Doi | 10.1016/j.bbrc.2016.05.160 |
| Citation | Uchida A, et al. (2016) In vivo dynamics of GFRalpha1-positive spermatogonia stimulated by GDNF signals using a bead transplantation assay. Biochem Biophys Res Commun 476(4):546-52 |
| abstractText | In mouse testes, spermatogonial stem cells (SSCs), a subpopulation of GFRalpha1 (GDNF family receptor-alpha1)-positive spermatogonia, are widely distributed along the convoluted seminiferous tubules. The proliferation and differentiation of the SSCs are regulated in part by local expression of GDNF (glial cell-derived neurotorphic factor), one of major niche factors for SSCs. However, the in vivo dynamics of the GDNF-stimulated GFRalpha1-positive spermatogonia remains unclear. Here, we developed a simple method for transplanting DiI-labeled and GDNF-soaked beads into the mouse testicular interstitium. By using this method, we examined the dynamics of GFRalpha1-positive spermatogonia in the tubular walls close to the transplanted GDNF-soaked beads. The bead-derived GDNF signals were able to induce the stratified aggregate formation of GFRalpha1-positive undifferentiated spermatogonia by day 3 post-transplantation. Each aggregate consisted of tightly compacted Asingle and marginal Apaired-Aaligned GFRalpha1-positive spermatogonia and was surrounded by Aaligned GFRalpha1-negative spermatogonia at more advanced stages. These data not only provide in vivo evidence for the inductive roles of GDNF in forming a rapid aggregation of GFRalpha1-positive spermatogonia but also indicate the usefulness of this in vivo assay system of various growth factors for the stem/progenitor spermatogonia in mammalian spermatogenesis. |
