| First Author | Pang ZP | Year | 2006 |
| Journal | EMBO J | Volume | 25 |
| Issue | 10 | Pages | 2039-50 |
| PubMed ID | 16642042 | Mgi Jnum | J:108786 |
| Mgi Id | MGI:3624891 | Doi | 10.1038/sj.emboj.7601103 |
| Citation | Pang ZP, et al. (2006) Genetic analysis of synaptotagmin 2 in spontaneous and Ca(2+)-triggered neurotransmitter release. EMBO J 25(10):2039-50 |
| abstractText | Synaptotagmin 2 resembles synaptotagmin 1, the Ca(2+) sensor for fast neurotransmitter release in forebrain synapses, but little is known about synaptotagmin 2 function. Here, we describe a severely ataxic mouse strain that harbors a single, destabilizing amino-acid substitution (I377N) in synaptotagmin 2. In Calyx of Held synapses, this mutation causes a delay and a decrease in Ca(2+)-induced but not in hypertonic sucrose-induced release, suggesting that synaptotagmin 2 mediates Ca(2+) triggering of evoked release in brainstem synapses. Unexpectedly, we additionally observed in synaptotagmin 2 mutant synapses a dramatic increase in spontaneous release. Synaptotagmin 1-deficient excitatory and inhibitory cortical synapses also displayed a large increase in spontaneous release, demonstrating that this effect was shared among synaptotagmins 1 and 2. Our data suggest that synaptotagmin 1 and 2 perform equivalent functions in the Ca(2+) triggering of action potential-induced release and in the restriction of spontaneous release, consistent with a general role of synaptotagmins in controlling 'release slots' for synaptic vesicles at the active zone. |
