| First Author | Chandran RR | Year | 2024 |
| Journal | Front Cell Dev Biol | Volume | 12 |
| Pages | 1335061 | PubMed ID | 38572485 |
| Mgi Jnum | J:352304 | Mgi Id | MGI:7619244 |
| Doi | 10.3389/fcell.2024.1335061 | Citation | Chandran RR, et al. (2024) Dedifferentiated early postnatal lung myofibroblasts redifferentiate in adult disease. Front Cell Dev Biol 12:1335061 |
| abstractText | Alveolarization ensures sufficient lung surface area for gas exchange, and during bulk alveolarization in mice (postnatal day [P] 4.5-14.5), alpha-smooth muscle actin (SMA)(+) myofibroblasts accumulate, secrete elastin, and lay down alveolar septum. Herein, we delineate the dynamics of the lineage of early postnatal SMA(+) myofibroblasts during and after bulk alveolarization and in response to lung injury. SMA(+) lung myofibroblasts first appear at approximately P2.5 and proliferate robustly. Lineage tracing shows that, at P14.5 and over the next few days, the vast majority of SMA(+) myofibroblasts downregulate smooth muscle cell markers and undergo apoptosis. Of note, approximately 8% of these dedifferentiated cells and another approximately 1% of SMA(+) myofibroblasts persist to adulthood. Single cell RNA sequencing analysis of the persistent SMA(-) cells and SMA(+) myofibroblasts in the adult lung reveals distinct gene expression profiles. For instance, dedifferentiated SMA(-) cells exhibit higher levels of tissue remodeling genes. Most interestingly, these dedifferentiated early postnatal myofibroblasts re-express SMA upon exposure of the adult lung to hypoxia or the pro-fibrotic drug bleomycin. However, unlike during alveolarization, these cells that re-express SMA do not proliferate with hypoxia. In sum, dedifferentiated early postnatal myofibroblasts are a previously undescribed cell type in the adult lung and redifferentiate in response to injury. |
