| First Author | Lindström NO | Year | 2018 |
| Journal | Dev Cell | Volume | 45 |
| Issue | 5 | Pages | 651-660.e4 |
| PubMed ID | 29870722 | Mgi Jnum | J:284158 |
| Mgi Id | MGI:6390022 | Doi | 10.1016/j.devcel.2018.05.010 |
| Citation | Lindstrom NO, et al. (2018) Progressive Recruitment of Mesenchymal Progenitors Reveals a Time-Dependent Process of Cell Fate Acquisition in Mouse and Human Nephrogenesis. Dev Cell 45(5):651-660.e4 |
| abstractText | Mammalian nephrons arise from a limited nephron progenitor pool through a reiterative inductive process extending over days (mouse) or weeks (human) of kidney development. Here, we present evidence that human nephron patterning reflects a time-dependent process of recruitment of mesenchymal progenitors into an epithelial nephron precursor. Progressive recruitment predicted from high-resolution image analysis and three-dimensional reconstruction of human nephrogenesis was confirmed through direct visualization and cell fate analysis of mouse kidney organ cultures. Single-cell RNA sequencing of the human nephrogenic niche provided molecular insights into these early patterning processes and predicted developmental trajectories adopted by nephron progenitor cells in forming segment-specific domains of the human nephron. The temporal-recruitment model for nephron polarity and patterning suggested by direct analysis of human kidney development provides a framework for integrating signaling pathways driving mammalian nephrogenesis. |
