| First Author | Xu J | Year | 2017 |
| Journal | Mol Reprod Dev | Volume | 84 |
| Issue | 3 | Pages | 257-264 |
| PubMed ID | 28029195 | Mgi Jnum | J:263747 |
| Mgi Id | MGI:6192983 | Doi | 10.1002/mrd.22772 |
| Citation | Xu J, et al. (2017) Expression of tamoxifen-inducible CRE recombinase in Lcn5-CreER(T2) transgenic mouse caput epididymis. Mol Reprod Dev 84(3):257-264 |
| abstractText | The epididymis, which connects the testis to vas deferens, plays a crucial role regulating sperm maturation and fertilization. Here, a tamoxifen-inducible CreER(T2) recombinase transgenic mouse was generated to study the function of genes in the caput epididymis using the Cre/LoxP system, which is driven by the 1.8-kb Lcn5 promoter (Lcn5-CreER(T2) ). Both CRE recombinase and ER(T2) mRNA were specifically expressed in the caput epididymis, beginning at postnatal Day 30 and increasing thereafter. Crossing these Lcn5-CreER(T2) transgenic mice with Rosa26; mT/mG reporter mice, which express membrane-bound GFP (mGFP) only after CRE is active at its genetic locus, resulted in the presence of GFP only in the middle/distal caput epididymis after tamoxifen induction. Efficiency of the CRE recombinase production in the caput epididymis was dose- and time-dependent. These tamoxifen-inducible caput epididymis-specific CRE recombinase transgenic mice thus provides a simple approach to modulate epididymal principal cells in vivo, allowing for the genetic investigation of caput epididymis-specific gene functions during sperm maturation. 84: 257-264, 2017. (c) 2016 Wiley Periodicals, Inc. |
