| First Author | Gommers LMM | Year | 2019 |
| Journal | PLoS One | Volume | 14 |
| Issue | 6 | Pages | e0217925 |
| PubMed ID | 31163064 | Mgi Jnum | J:279232 |
| Mgi Id | MGI:6315131 | Doi | 10.1371/journal.pone.0217925 |
| Citation | Gommers LMM, et al. (2019) Low extracellular magnesium does not impair glucose-stimulated insulin secretion. PLoS One 14(6):e0217925 |
| abstractText | There is an increasing amount of clinical evidence that hypomagnesemia (serum Mg2+ levels < 0.7 mmol/l) contributes to type 2 diabetes mellitus pathogenesis. Amongst other hypotheses, it has been suggested that Mg2+ deficiency affects insulin secretion. The aim of this study was, therefore, to investigate the acute effects of extracellular Mg2+ on glucose-stimulated insulin secretion in primary mouse islets of Langerhans and the rat insulinoma INS-1 cell line. Here we show that acute lowering of extracellular Mg2+ concentrations from 1.0 mM to 0.5 mM did not affect glucose-stimulated insulin secretion in islets or in insulin-secreting INS-1 cells. The expression of key genes in the insulin secretory pathway (e.g. Gck, Abcc8) was also unchanged in both experimental models. Knockdown of the most abundant Mg2+ channel Trpm7 by siRNAs in INS-1 cells resulted in a 3-fold increase in insulin secretion at stimulatory glucose conditions compared to mock-transfected cells. Our data suggest that insulin secretion is not affected by acute lowering of extracellular Mg2+ concentrations. |
