| Primary Identifier | MGI:3722188 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Pgk1-FGF2)48Cofn |
| Strain of Origin | FVB/N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Human FGF2 cDNA including the coding sequences for all three CUG and the AUG translational start coding sequences was put under control of a constitutive phosphoglycerate kinase promoter. The Pgk1 polyadenylation sequence was also included in the construct. The construct was microinjected into pronuclear FVB/N oocytes. Production of human FGF2 protein was verified by Western blot. Founder lines with stable copy number and transmission of the transgene were identified and line 48 was established. |
