| Primary Identifier | MGI:3722867 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mrc2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to replace exons 2-5 and part of exon 6 with a neomycin resistance cassette. Analysis of mutant MEFs determined that a transcript is produced, and sequencing showed it resulted from exon 1 being spliced to exon 7, with a continuous open reading frame. A protein lacking the cysteine-rich domain (exon 2), the FNII domain (exon 3) and the first CTLD (exons 4-6) was expressed. |
