Primary Identifier | MGI:3723519 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Tamalin |
Transmission | Germline | Strain of Origin | 129S/SvEv-Gpi1<c> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Using a targeting vector constructed with 129/Sv mouse DNA, a floxed neo cassette was inserted upstream of exon 5 and an additional lox P site was inserted downstream of exon 7. Cre-mediated recombination removed the neo cassette and exons 5 through 7 to create a truncated protein detectable by western blot analysis that lacks a complete PDZ dmain and leucine zipper. |