| First Author | Nabi D | Year | 2021 |
| Journal | Nat Commun | Volume | 12 |
| Issue | 1 | Pages | 6547 |
| PubMed ID | 34764261 | Mgi Jnum | J:314905 |
| Mgi Id | MGI:6826763 | Doi | 10.1038/s41467-021-26826-3 |
| Citation | Nabi D, et al. (2021) CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes. Nat Commun 12(1):6547 |
| abstractText | Proper chromosome segregation is essential to avoid aneuploidy, yet this process fails with increasing age in mammalian oocytes. Here we report a role for the scarcely described protein CENP-V in oocyte spindle formation and chromosome segregation. We show that depending on the oocyte maturation state, CENP-V localizes to centromeres, to microtubule organizing centers, and to spindle microtubules. We find that Cenp-V(-/-) oocytes feature severe deficiencies, including metaphase I arrest, strongly reduced polar body extrusion, increased numbers of mis-aligned chromosomes and aneuploidy, multipolar spindles, unfocused spindle poles and loss of kinetochore spindle fibres. We also show that CENP-V protein binds, diffuses along, and bundles microtubules in vitro. The spindle assembly checkpoint arrests about half of metaphase I Cenp-V(-/-) oocytes from young adults only. This finding suggests checkpoint weakening in ageing oocytes, which mature despite carrying mis-aligned chromosomes. Thus, CENP-V is a microtubule bundling protein crucial to faithful oocyte meiosis, and Cenp-V(-/-) oocytes reveal age-dependent weakening of the spindle assembly checkpoint. |
