| First Author | de Castro RO | Year | 2022 |
| Journal | Development | Volume | 149 |
| Issue | 18 | PubMed ID | 36111709 |
| Mgi Jnum | J:329538 | Mgi Id | MGI:7344013 |
| Doi | 10.1242/dev.199967 | Citation | de Castro RO, et al. (2022) PBAF chromatin remodeler complexes that mediate meiotic transitions in mouse. Development 149(18):dev199967 |
| abstractText | Gametogenesis in mammals encompasses highly regulated developmental transitions. These are associated with changes in transcription that cause characteristic patterns of gene expression observed during distinct stages of gamete development, which include specific activities with critical meiotic functions. SWI/SNF chromatin remodelers are recognized regulators of gene transcription and DNA repair, but their composition and functions in meiosis are poorly understood. We have generated gamete-specific conditional knockout mice for ARID2, a specific regulatory subunit of PBAF, and have compared its phenotype with BRG1 knockouts, the catalytic subunit of PBAF/BAF complexes. While Brg1Delta/Delta knockout acts at an early stage of meiosis and causes cell arrest at pachynema, ARID2 activity is apparently required at the end of prophase I. Striking defects in spindle assembly and chromosome-spindle attachment observed in Arid2Delta/Delta knockouts are attributed to an increase in aurora B kinase, a master regulator of chromosome segregation, at centromeres. Further genetic and biochemical analyses suggest the formation of a canonical PBAF and a BRG1-independent complex containing ARID2 and PBRM1 as core components. The data support a model in which different PBAF complexes regulate different stages of meiosis and gametogenesis. |
