| First Author | David CK | Year | 2022 |
| Journal | Sci Data | Volume | 9 |
| Issue | 1 | Pages | 457 |
| PubMed ID | 35907922 | Mgi Jnum | J:346636 |
| Mgi Id | MGI:7617183 | Doi | 10.1038/s41597-022-01569-y |
| Citation | David CK, et al. (2022) Single cell transcriptome sequencing of inspiratory neurons of the preBotzinger complex in neonatal mice. Sci Data 9(1):457 |
| abstractText | Neurons in the brainstem preBotzinger complex (preBotC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory neurons in the preBotC of neonatal mouse slices that retain breathing-related rhythmicity in vitro. We classified neurons based on their electrophysiological properties and genetic background, and then aspirated their cellular contents for single-cell RNA sequencing (scRNA-seq). This data set provides the raw nucleotide sequences (FASTQ files) and annotated files of nucleotide sequences mapped to the mouse genome (mm10 from Ensembl), which includes the fragment counts, gene lengths, and fragments per kilobase of transcript per million mapped reads (FPKM). These data reflect the transcriptomes of the neurons that generate the rhythm and pattern for inspiratory breathing movements. |
