| First Author | Stone JS | Year | 2018 |
| Journal | J Assoc Res Otolaryngol | Volume | 19 |
| Issue | 4 | Pages | 381-399 |
| PubMed ID | 29869046 | Mgi Jnum | J:270875 |
| Mgi Id | MGI:6279590 | Doi | 10.1007/s10162-018-0676-6 |
| Citation | Stone JS, et al. (2018) Characterization of Adult Vestibular Organs in 11 CreER Mouse Lines. J Assoc Res Otolaryngol 19(4):381-399 |
| abstractText | Utricles are vestibular sense organs that encode linear head movements. They are composed of a sensory epithelium with type I and type II hair cells and supporting cells, sitting atop connective tissue, through which vestibular nerves project. We characterized utricular Cre expression in 11 murine CreER lines using the ROSA26(tdTomato) reporter line and tamoxifen induction at 6 weeks of age. This characterization included Calbindin2(CreERT2), Fgfr3-iCreER(T2), GFAP-A-CreER, GFAP-B-CreER, GLAST-CreER(T2), Id2(CreERT2), Otoferlin(CreERT2), Parvalbumin(CreERT2), Prox1(CreERT2), Sox2(CreERT2), and Sox9-CreER(T2). Otoferlin(CreERT2) mice had inducible Cre activity specific to hair cells. GLAST-CreER(T2), Id2(CreERT2), and Sox9-CreER(T2) had inducible Cre activity specific to supporting cells. Sox2(CreERT2) had inducible Cre activity in supporting cells and most type II hair cells. Parvalbumin(CreERT2) mice had small numbers of labeled vestibular nerve afferents. Calbindin2(CreERT2) mice had labeling of most type II hair cells and some type I hair cells and supporting cells. Only rare (or no) tdTomato-positive cells were detected in utricles of Fgfr3-iCreER(T2), GFAP-A-CreER, GFAP-B-CreER, and Prox1(CreERT2) mice. No Cre leakiness (tdTomato expression in the absence of tamoxifen) was observed in Otoferlin(CreERT2) mice. A small degree of leakiness was seen in GLAST-CreER(T2), Id2(CreERT2), Sox2(CreERT2), and Sox9-CreER(T2) lines. Calbindin2(CreERT2) mice had similar tdTomato expression with or without tamoxifen, indicating lack of inducible control under the conditions tested. In conclusion, 5 lines-GLAST-CreER(T2), Id2(CreERT2), Otoferlin(CreERT2), Sox2(CreERT2), and Sox9-CreER(T2)-showed cell-selective, inducible Cre activity with little leakiness, providing new genetic tools for researchers studying the vestibular periphery. |
