| Primary Identifier | MGI:3760243 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rapgef2 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:125199 does not mention whether the null allele of Pou5f1 is also present in the line of mice created. |
| molecularNote | Exon 15 was removed by cre-mediated recombination following a cross with Tg(ACTB-cre)13Miya mice. Removal of the exon was confirmed by PCR and absence of a protein product was confirmed by western blot analysis on proteins extracted from E10.5 embryos. |
