| First Author | Fuerst PG | Year | 2010 |
| Journal | Genesis | Volume | 48 |
| Issue | 10 | Pages | 578-84 |
| PubMed ID | 20715164 | Mgi Jnum | J:163979 |
| Mgi Id | MGI:4830357 | Doi | 10.1002/dvg.20662 |
| Citation | Fuerst PG, et al. (2010) A novel null allele of mouse DSCAM survives to adulthood on an inbred C3H background with reduced phenotypic variability. Genesis 48(10):578-84 |
| abstractText | DSCAMs are cell adhesion molecules that play several important roles in neurodevelopment. Mouse alleles of Dscam identified to date do not survive on an inbred C57BL/6 background, complicating analysis of DSCAM-dependent developmental processes because of phenotypic variability related to the segregating backgrounds needed for postnatal survival. A novel spontaneous allele of Dscam, hereafter referred to as Dscam(2)(J), has been identified. This allele contains a four base pair duplication in exon 19, leading to a frameshift and truncation of the open reading frame. Mice homozygous for the Dscam(2)(J) mutant allele survive into adulthood on the C3H/HeJ background on which the mutation was identified. Using the Dscam(2)(J) allele, retinal phenotypes that have variable severity on a segregating background were examined. A neurite lamination defect similar to that described in chick was discovered in mice. These results indicate that, in the retina, additional DSCAM-dependent processes can be found by analysis of mutations on different genetic backgrounds. |
