| First Author | Kuma A | Year | 2008 |
| Journal | Autophagy | Volume | 4 |
| Issue | 1 | Pages | 61-2 |
| PubMed ID | 17786029 | Mgi Jnum | J:276483 |
| Mgi Id | MGI:6316359 | Doi | 10.4161/auto.4846 |
| Citation | Kuma A, et al. (2008) Chromosomal mapping of the GFP-LC3 transgene in GFP-LC3 mice. Autophagy 4(1):61-2 |
| abstractText | GFP-LC3 mice are widely used to monitor autophagy. Here, we report chromosomal mapping of the GFP.LC3 transgene in this mouse model. The transgene was located in a very distal region of chromosome 2, immediately upstream of a putative open reading frame that is thought to be a part of a pseudogene. With this information, we were able to generate a PCR system to easily determine zygosity. Homozygous GFP-LC3 mice (GFP-LC3(tg/tg)) were healthy, fertile and did not show an abnormal phenotype. Thus, GFP-LC3 integration in this model appears not to affect functions of other genes, justifying use of this mouse model. |
