Primary Identifier | MGI:3762002 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Del(11Hoxb3-Hoxb4)1Karl |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Floxed neo cassettes were inserted upstream of Hoxb4 and downstream of Hoxb3. Cre mediated recombination using a transiently expressed cre plasmid was used to create a deletion region that encompasses both genes. The absence of either transcript was confirmed by RT-PCR using RNA extracted from bone marrow cells. |