| First Author | Abdi K | Year | 2018 |
| Journal | J Immunol | Volume | 201 |
| Issue | 10 | Pages | 2879-2884 |
| PubMed ID | 30315139 | Mgi Jnum | J:267970 |
| Mgi Id | MGI:6258175 | Doi | 10.4049/jimmunol.1800721 |
| Citation | Abdi K, et al. (2018) Cutting Edge: Quantitative Determination of CD40L Threshold for IL-12 and IL-23 Production from Dendritic Cells. J Immunol 201(10):2879-2884 |
| abstractText | Early secretion of IL-12 by mouse dendritic cells (DCs) instructs T cells to make IFN-gamma. However, only activated, but not naive T cells are able to license DCs for IL-12 production. We hypothesized that it might be due to different levels of CD40L expression on the surface of these cells, as CD40 signals are required for IL-12 production. Using quantitative cell-free systems incorporating CD40L in lipid bilayers combined with total internal reflection fluorescence microscopy and flow cytometry, we show that as low as approximately 200 CD40L molecules/mum(2) in combination with IL-4 is sufficient to induce IL-12 production by DCs. Remarkably, CD40L alone is adequate to induce IL-23 secretion by DCs. Thus, although activated T cells have somewhat higher levels of CD40L, it is the combination of CD40L and the cytokines they secrete that licenses DCs and influences the effector class of the immune response. |
