| First Author | Khan SN | Year | 2008 |
| Journal | Proc Natl Acad Sci U S A | Volume | 105 |
| Issue | 10 | Pages | 3861-6 |
| PubMed ID | 18310318 | Mgi Jnum | J:132861 |
| Mgi Id | MGI:3777059 | Doi | 10.1073/pnas.0800025105 |
| Citation | Khan SN, et al. (2008) Editing and escape from editing in anti-DNA B cells. Proc Natl Acad Sci U S A 105(10):3861-6 |
| abstractText | Tolerance to dsDNA is achieved through editing of Ig receptors that react with dsDNA. Nevertheless, some B cells with anti-dsDNA receptors escape editing and migrate to the spleen. Certain anti-dsDNA B cells that are recovered as hybridomas from the spleens of anti-dsDNA H chain transgenic mice also bind an additional, Golgi-associated antigen. B cells that bind this antigen accumulate intracellular IgM. The intracellular accumulation of IgM is incomplete, because IgM clusters are observed at the cell surface. In the spleen, B cells that express the heavy and light chains encoding this IgM are surface IgM-bright and acquire the CD21-high/CD23-low phenotype of marginal zone B cells. Our data imply that expression of an Ig that binds dsDNA and an additional antigen expressed in the secretory compartment renders B cells resistant to central tolerance. In the periphery, these B cells may be sequestered in the splenic marginal zone. |
