| First Author | Hu Z | Year | 2018 |
| Journal | Front Neurosci | Volume | 12 |
| Pages | 465 | PubMed ID | 30050402 |
| Mgi Jnum | J:275907 | Mgi Id | MGI:6307232 |
| Doi | 10.3389/fnins.2018.00465 | Citation | Hu Z, et al. (2018) Silencing miR-150 Ameliorates Experimental Autoimmune Encephalomyelitis. Front Neurosci 12:465 |
| abstractText | MiR-150 regulates maturation and differentiation of T cells but how it functions in multiple sclerosis (MS) is unclear. In miR-150 knockout (KO) mice, we examined the effect of miR-150 deletion on disease severity of experimental autoimmune encephalomyelitis (EAE), an animal model of MS. After deleting miR-150, EAE disease severity was reduced according to clinical score. Histological staining and MBP immunofluorescence staining revealed that miR-150 deletion limited the extent of inflammatory demyelination and axonal damage in the spinal cord. Flow cytometry showed that CD3(+), CD4(+), and CD8(+) T cells were increased in WT-EAE mice, but miR-150 deletion significantly reversed EAE-mediated up-regulation of CD3(+), CD4(+), and CD8(+) T cells and down-regulation of CD19(+) B cells. In addition, miR-150 deletion reduced the mRNA expression of IL-1beta, IL-6, IL-17, and TNF-alpha in spleen and spinal cord after EAE induction. Thus, miR-150 deletion reduces EAE severity and demyelination, probably through inhibiting the activated immune response and the inflammation in the central nervous system. |
