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Allele : Tg(CAG-Serpine1)1Dgi transgene insertion 1, David Ginsburg
Primary Identifier  MGI:3763900 Allele Type  Transgenic
Attribute String  Inserted expressed sequence Gene  Tg(CAG-Serpine1)1Dgi
Strain of Origin  (C57BL/6 x SJL)F1 Is Recombinase  false
Is Wild Type  false
molecularNote  Oligo-based site-directed mutagenesis was use to mutate codon 177 from AAG to ACC (K154T), and codon 342 from CAG to CTG (Q319L) in the mature mouse protein. Expression is driven by the CAG promoter (the chicken beta-actin (Actb) promoter with a CMV immediate-early enhancer). The 2 amino acid substitutions more than triple the functional half-life of the protein and expression is increased approximately 170-fold over wildtype. Quantitative PCR reveals elevated expression in brain, heart, lung, liver, spleen, intestine, kidney, fat, skin, and muscle (strongest in liver, brain, heart and kidney). Immunohistochemistry reveals expression in kidney, liver, spleen, and lung.
  • mutations:
  • Insertion
  • synonyms:
  • PAI1 transgenic (actin promoter),
  • PAI-1 line 1,
  • PAI-1 line 1,
  • Tg(Actb-Serpine1)1Dgi,
  • PAI1 transgenic (actin promoter),
  • Tg(Actb-Serpine1)1Dgi
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