| First Author | Mujalli A | Year | 2018 |
| Journal | Biochim Biophys Acta Mol Cell Biol Lipids | Volume | 1863 |
| Issue | 9 | Pages | 1121-1131 |
| PubMed ID | 29902570 | Mgi Jnum | J:266612 |
| Mgi Id | MGI:6199827 | Doi | 10.1016/j.bbalip.2018.06.009 |
| Citation | Mujalli A, et al. (2018) Profiling of phosphoinositide molecular species in human and mouse platelets identifies new species increasing following stimulation. Biochim Biophys Acta Mol Cell Biol Lipids 1863(9):1121-1131 |
| abstractText | Phosphoinositides are bioactive lipids essential in the regulation of cell signaling as well as cytoskeleton and membrane dynamics. Their metabolism is highly active in blood platelets where they play a critical role during activation, at least through two well identified pathways involving phospholipase C and phosphoinositide 3-kinases (PI3K). Here, using a sensitive high-performance liquid chromatography-mass spectrometry method recently developed, we monitored for the first time the profiling of phosphatidylinositol (PI), PIP, PIP2 and PIP3 molecular species (fatty-acyl profiles) in human and mouse platelets during the course of stimulation by thrombin and collagen-related peptide. Furthermore, using class IA PI3K p110alpha or p110beta deficient mouse platelets and a pharmacological inhibitor, we show the crucial role of p110beta and the more subtle role of p110alpha in the production of PIP3 molecular species following stimulation. This comprehensive platelet phosphoinositides profiling provides important resources for future studies and reveals new information on phosphoinositides biology, similarities and differences in mouse and human platelets and unexpected dramatic increase in low-abundance molecular species of PIP2 during stimulation, opening new perspectives in phosphoinositide signaling in platelets. |
