| Primary Identifier | MGI:3764984 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tpt1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed cassette containing both Neo and TK selection markers was introduced into the second intron and a third loxP site into intron 4 of the gene. After transient Cre expression, clones were selected that had the selection cassette and exons 3 and 4 removed. Gene inactivation was confirmed by immunoblotting of protein from E9.5 embryos. |