Primary Identifier | MGI:3765235 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Cd4 |
Transmission | Germline | Strain of Origin | 129S2/SvPas |
Is Recombinase | false | Is Wild Type | false |
molecularNote | ES cells containing a previously targeted Lag3 allele (Lag3tm1Doi) were targeted again with a vector replacing part of exon 5 of the Cd4 gene with a hygromycin selection cassette. Detection of doubly mutant embryos was determined by PCR and Southern blotting. |