| Primary Identifier | MGI:3764832 | Allele Type | Transgenic |
| Attribute String | Hypomorph, Inserted expressed sequence | Gene | Epm2a |
| Strain of Origin | (C57BL/6J x CBA/J)F2 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The transgenic mice express rearranged Valpha and Vbeta genes from a CTL clone that recognized the SV40 large T antigen (SV-T). The V and J regions of a transgene construct (made in J:107523) were replaced with the V and J regions of the clone K T cell receptor. The synthetic alpha and beta constructs were coinjected into 1 day embryos. Three lines were obtained and line TG-B was further characterized. The transgene in line TG-B inserted into intron 1. Western blot analysis confirmed reduced protein expression. |
