| First Author | Huang K | Year | 2023 |
| Journal | ACS Sens | Volume | 8 |
| Issue | 2 | Pages | 767-773 |
| PubMed ID | 36689294 | Mgi Jnum | J:355531 |
| Mgi Id | MGI:7719059 | Doi | 10.1021/acssensors.2c02317 |
| Citation | Huang K, et al. (2023) Base-Exchange Enabling the Visualization of SARM1 Activities in Sciatic Nerve-Injured Mice. ACS Sens 8(2):767-773 |
| abstractText | Enzymes are important in homeostasis in living organisms. Since abnormal enzyme activities are highly associated with many human diseases, detection of in vivo activities of a specific enzyme is important to study the pathology of the related diseases. In this work, we have designed and synthesized a series of new small-molecule-activatable fluorescent probes for the imaging of Sterile Alpha and TIR Motif-containing 1 (SARM1) activities based on its transglycosidase activities (base-exchange reactions of NAD(+)). Probe 1a was found to undergo base-exchange reactions with NAD(+) in the presence of activated SARM1 but not CD38 nor NADase and formed a highly emissive product AD-1a [about a 100-fold fluorescence enhancement in 20 min with a 150 nm (5665 cm(-1)) Stokes shift and a 100 nm (3812 cm(-1)) red shift]. This probe exhibited a higher reactivity and sensitivity than those commonly used for SARM1 imaging. The utilities of 1a have also been demonstrated in live-cell imaging and detection of in vivo activities of SARM1 in a sciatic nerve injury mouse model. |
