| Primary Identifier | MGI:3767564 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Actn4 |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A Lys256Glu mutation, such as is associated with familial focal and segmental glomerulosclerosis (FSGS) was introduced into exon 8. In addition, a loxP flanked neomycin-resistance cassette was inserted 400 base pairs 3' of exon 8. The neomycin resistance cassette was removed by mating mice with Tg(Prm-cre)58Og, resulting in transcript expression that is similar to the expression in wild-type, as indicated by Northern blot. RT-PCR and sequencing of exon 8 confirmed that the transcript contains the point mutation, K228E. |
