| Primary Identifier | MGI:3767825 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(DBH-RET*M918T)CH7214Rpk |
| Strain of Origin | (DBA x C57BL/6)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A human cDNA encoding the short isoform (1072 amino acids) of RET was mutated by PCR to introduce the RET MEN2B mutation (Met918Thr). This mutated cDNA was inserted downstream of a human DBH promoter (5.8 kb). Between the transcriptional start site and the mutated cDNA, the construct contains the first intron of the rat preproinsulin gene (+13 ±+183 nt). A polyadenylation site from the murine protamine 1 gene was inserted at the 3' end. This transgenic line has a copy number of 260. |
