| Primary Identifier | MGI:3768641 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Abcg2 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP-flanked neomycin selection cassette and an IRES-GFP reporter gene were inserted immediately downstream of the stop codon and upstream of the endogenous polyadenylation signal sequence. Targeting took place in ES cells homozygous for the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells. Cre-mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. Reporter gene expression was confirmed by immunohistochemistry on intestine and kidney from homozygotes. |
