| First Author | He C | Year | 2018 |
| Journal | Biochem Biophys Res Commun | Volume | 504 |
| Issue | 4 | Pages | 899-902 |
| PubMed ID | 30224066 | Mgi Jnum | J:272380 |
| Mgi Id | MGI:6280731 | Doi | 10.1016/j.bbrc.2018.09.051 |
| Citation | He C, et al. (2018) NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes. Biochem Biophys Res Commun 504(4):899-902 |
| abstractText | Heterogeneity in the metabolic properties of adipocytes in white adipose tissue has been well documented. We sought to investigate metabolic heterogeneity in adipocytes of brown adipose tissue (BAT), focusing on heterogeneity in nutrient uptake. To explore the possibility of metabolic heterogeneity in brown adipocytes, we used nanoscale secondary ion mass spectrometry (NanoSIMS) to quantify uptake of lipids in adipocytes interscapular BAT and perivascular adipose tissue (PVAT) after an intravenous injection of triglyceride-rich lipoproteins (TRLs) containing [(2)H]triglycerides ((2)H-TRLs). The uptake of deuterated lipids into brown adipocytes was quantified by NanoSIMS. We also examined (13)C enrichment in brown adipocytes after administering [(13)C]glucose or (13)C-labeled mixed fatty acids by gastric gavage. The uptake of (2)H-TRLs-derived lipids into brown adipocytes was heterogeneous, with (2)H enrichment in adjacent adipocytes varying by more than fourfold. We also observed substantial heterogeneity in (13)C enrichment in adjacent brown adipocytes after administering [(13)C]glucose or [(13)C]fatty acids by gastric gavage. The uptake of nutrients by adjacent brown adipocytes within a single depot is variable, suggesting that there is heterogeneity in the metabolic properties of brown adipocytes. |
